tyrA [2019-08-07 08:13:20]

prephenate dehydrogenase

Locus: BSU_22610

Isoelectric point: 5.47

Molecular weight: 41.28 kDa

Protein length: 371 aa

Gene length: 1116 bp

Function: biosynthesis of tyrosine

Product: prephenate dehydrogenase

Essential: no

E.C.: 1.3.1.12

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids

Gene

Coordinates: 2,369,251 → 2,370,366

The protein

Catalyzed reaction/ biological activity

• Prephenate + NAD⁺ = 4-hydroxyphenylpyruvate + CO₂ + NADH (according to UniProt)

Protein family

• prephenate/arogenate dehydrogenase family (single member, according to UniProt)

Domains

• ACT domain (aa 300 ... 371) (according to the Interpro database)

Cofactors

• NAD⁺ (according to UniProt)

Effectors of protein activity

• subject to feedback inhibition by tyrosine PubMed

Structure

• 3DZB (from Streptococcus thermophilus, 42% identity)

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE
  Description: PubMed

  Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

• Genes: hisC-tyrA-aroE
  Description: PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• GP2358 ∆tyrA::kan, available in Jörg Stülke's lab
• BKE22610 (ΔtyrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACGTCATCACCTGGTTT, downstream forward: _UP4_TTTTATGCTGATTGAGGTGG
• BKK22610 (ΔtyrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACGTCATCACCTGGTTT, downstream forward: _UP4_TTTTATGCTGATTGAGGTGG

References

Reviews

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Original publications

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